Immunofluorescence assay for serologic diagnosis of SARS.
Identifieur interne : 005304 ( Main/Exploration ); précédent : 005303; suivant : 005305Immunofluorescence assay for serologic diagnosis of SARS.
Auteurs : Paul K S. Chan [République populaire de Chine] ; King-Cheung Ng ; Rickjason C W. Chan ; Rebecca K Y. Lam ; Viola C Y. Chow ; Mamie Hui ; Alan Wu ; Nelson Lee ; Florence H Y. Yap ; Frankie W T. Cheng ; Joseph J Y. Sung ; John S. TamSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Anticorps antiviraux (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Hong Kong, Humains, Maladies transmissibles émergentes (diagnostic), Maladies transmissibles émergentes (immunologie), Maladies transmissibles émergentes (physiopathologie), Mâle, RT-PCR, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (physiopathologie), Technique d'immunofluorescence indirecte ().
- MESH :
- diagnostic : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- immunologie : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- isolement et purification : Anticorps antiviraux.
- physiopathologie : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- Adolescent, Adulte, Adulte d'âge moyen, Enfant, Enfant d'âge préscolaire, Femelle, Hong Kong, Humains, Mâle, RT-PCR, Technique d'immunofluorescence indirecte.
- Wicri :
- geographic : Hong Kong.
English descriptors
- KwdEn :
- Adolescent, Adult, Antibodies, Viral (isolation & purification), Child, Child, Preschool, Communicable Diseases, Emerging (diagnosis), Communicable Diseases, Emerging (immunology), Communicable Diseases, Emerging (physiopathology), Female, Fluorescent Antibody Technique, Indirect (methods), Hong Kong, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (physiopathology).
- MESH :
- chemical , isolation & purification : Antibodies, Viral.
- geographic : Hong Kong.
- diagnosis : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- immunology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- methods : Fluorescent Antibody Technique, Indirect.
- physiopathology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
DOI: 10.3201/eid1003.030493
PubMed: 15109430
Affiliations:
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Le document en format XML
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Chan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name></author><author><name sortKey="Chan, Rickjason C W" sort="Chan, Rickjason C W" uniqKey="Chan R" first="Rickjason C W" last="Chan">Rickjason C W. 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Chan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name></author><author><name sortKey="Chan, Rickjason C W" sort="Chan, Rickjason C W" uniqKey="Chan R" first="Rickjason C W" last="Chan">Rickjason C W. Chan</name></author><author><name sortKey="Lam, Rebecca K Y" sort="Lam, Rebecca K Y" uniqKey="Lam R" first="Rebecca K Y" last="Lam">Rebecca K Y. Lam</name></author><author><name sortKey="Chow, Viola C Y" sort="Chow, Viola C Y" uniqKey="Chow V" first="Viola C Y" last="Chow">Viola C Y. Chow</name></author><author><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name></author><author><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name></author><author><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name></author><author><name sortKey="Yap, Florence H Y" sort="Yap, Florence H Y" uniqKey="Yap F" first="Florence H Y" last="Yap">Florence H Y. Yap</name></author><author><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. 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All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><noCountry><name sortKey="Chan, Rickjason C W" sort="Chan, Rickjason C W" uniqKey="Chan R" first="Rickjason C W" last="Chan">Rickjason C W. Chan</name><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name><name sortKey="Chow, Viola C Y" sort="Chow, Viola C Y" uniqKey="Chow V" first="Viola C Y" last="Chow">Viola C Y. Chow</name><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name><name sortKey="Lam, Rebecca K Y" sort="Lam, Rebecca K Y" uniqKey="Lam R" first="Rebecca K Y" last="Lam">Rebecca K Y. Lam</name><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name><name sortKey="Yap, Florence H Y" sort="Yap, Florence H Y" uniqKey="Yap F" first="Florence H Y" last="Yap">Florence H Y. Yap</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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